Histology of seeds: sprouting the neurons for diagnosis ofpoisoning

AUTHORS : Saiyed MZG, Jani CB
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Introduction: The regulated and recommended consumptionof different plants is for nutrition but, their irrational useeither by accident, suicide or homicide can lead to illness,disease or even death in certain cases and is considered aspoisoning. It is extremely difficult to detect the biologicalpoisons at the laboratories in the samples collected fromthe patient. Its physical characteristics and histologicalfeatures are useful in diagnosis. As per best of our knowledge,no database is available regarding the physical andhistological characteristics of routinely consumed toxic seedsin biomedical literature. An attempt is made to prepare apictorial database of physical & microscopic characteristicsof frequently consumed seeds of toxicological significancein Gujarat region. Materials and methods: Toxic seedscommonly encountered in poisoning cases, procured fromthe local shop, were subjected to conventional method oftissue processing at Histopathology laboratory. The slideswere examined under light microscope and photographswere captured. The photographs were processed in Microsoftoffice picture manager software and were reproduced withbetter resolution. Results: The images of the histologicalfeatures of different seeds were produced and thecharacteristic features were identified. Conclusion: Apictorial database of histology of toxic seeds commonlycausing poisoning in the Gujarat region is created. It issuggested to the Forensic Medicine departments in differentmedical colleges to carry out such exercise to create theirregional database. Better images of histological featurescan be produced by using standard method of processing oftoxic seeds in sophisticated Botanical laboratories & areaof Forensic Botany can be explored further.

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